Fluorescent assay and release profile 

The standard curve calculated and shown in Figure 5a, relates light absorbance of the Rhodamine-B dye to concentration. This relationship is then used to determine the solute concentration. The percentage of cumulative release of the dye from the microparticles was calculated by determining the mass of dye in the supernatant and dividing it by the total mass of dye loaded into the particles. The total mass of dye in the microparticles was previously determined by collecting and measuring the supernatant of the media when the particles were loaded. The mass of the dye in the release assay supernatant was determined by dividing the absorbance by the slope of the Rhodamine-B standard curve (29.138abs/conc), which gives the concentration. This concentration was multiplied by the volume of supernatant (1mL PBS) to obtain the mass of dye in the supernatant. The characterization and release of Rhodamine B was successfully created and can be used as an effective model for the release of antiviral agents. 

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Figure 5 (a & b) : (a) Standard Curve of Rhodamine-B, (b) Cumulative Release Profile of Rhodamine-B in Engineered Microparticles